Papers of Interest
Output from consortia
Output from the hCOMET COST action > Load more
DNA damage
Tice,R.R., Agurell,E., Anderson,D., Burlinson,B., Hartmann,A., Kobayashi,H., Miyamae,Y., Rojas,E., Ryu,J.C., and Sasaki,Y.F. (2000) Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing. Environmental and Molecular Mutagenesis, 35, 206-221.
DNA repair
Collins,A.R., Dusinská,M., Horváthová,E., Munro,E., Savio,M., and Stetina,R. (2001) Inter-individual differences in repair of DNA base oxidation, measured in vitro with the comet assay. Mutagenesis, 16, 297-301.
First reports
Ostling O, Johanson KJ. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem Biophys Res Commun. 1984 Aug 30;123(1):291-8.
Published protocols
Collins A, Møller P, Gajski G, Vodenková S, Abdulwahed A, Anderson D, Bankoglu EE, Bonassi S, Boutet-Robinet E, Brunborg G, Chao C, Cooke MS, Costa C, Costa S, Dhawan A, de Lapuente J, Bo' CD, Dubus J, Dusinska M, Duthie SJ, Yamani NE, Engelward B, Gaivão I, Giovannelli L, Godschalk R, Guilherme S, Gutzkow KB, Habas K, Hernández A, Herrero O, Isidori M, Jha AN, Knasmüller S, Kooter IM, Koppen G, Kruszewski M, Ladeira C, Laffon B, Larramendy M, Hégarat LL, Lewies A, Lewinska A, Liwszyc GE, de Cerain AL, Manjanatha M, Marcos R, Milić M, de Andrade VM, Moretti M, Muruzabal D, Novak M, Oliveira R, Olsen AK, Owiti N, Pacheco M, Pandey AK, Pfuhler S, Pourrut B, Reisinger K, Rojas E, Rundén-Pran E, Sanz-Serrano J, Shaposhnikov S, Sipinen V, Smeets K, Stopper H, Teixeira JP, Valdiglesias V, Valverde M, van Acker F, van Schooten FJ, Vasquez M, Wentzel JF, Wnuk M, Wouters A, Žegura B, Zikmund T, Langie SAS, Azqueta A. Measuring DNA modifications with the comet assay: a compendium of protocols. Nat Protoc. 2023 Mar;18(3):929-989. doi: 10.1038/s41596-022-00754-y
Vodenkova S, Azqueta A, Collins A, Dusinska M, Gaivão I, Møller P, Opattova A, Vodicka P, Godschalk RWL, Langie SAS. An optimized comet-based in vitro DNA repair assay to assess base and nucleotide excision repair activity. Nat Protoc. 2020 Dec;15(12):3844-3878. doi: 10.1038/s41596-020-0401-x
Møller P, Azqueta A, Boutet-Robinet E, Koppen G, Bonassi S, Milić M, Gajski G, Costa S, Teixeira JP, Costa Pereira C, Dusinska M, Godschalk R, Brunborg G, Gutzkow KB, Giovannelli L, Cooke MS, Richling E, Laffon B, Valdiglesias V, Basaran N, Del Bo' C, Zegura B, Novak M, Stopper H, Vodicka P, Vodenkova S, de Andrade VM, Sramkova M, Gabelova A, Collins A, Langie SAS. Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results. Nat Protoc. 2020 Dec;15(12):3817-3826. doi: 10.1038/s41596-020-0398-1
Azqueta A, Langie SA, Slyskova J, Collins AR. Measurement of DNA base and nucleotide excision repair activities in mammalian cells and tissues using the comet assay--a methodological overview. DNA Repair (Amst). 2013 Nov;12(11):1007-10. doi: 10.1016/j.dnarep.2013.07.011
L. María Sierra and Isabel Gaivão (eds.), Genotoxicity and DNA Repair: A Practical Approach, Methods in Pharmacology and Toxicology, doi: 10.1007/978-1-4939-1068-7_1, © Springer Science + Business Media New York 2014
Books & Book chapters
L. María Sierra and Isabel Gaivão (eds.), Genotoxicity and DNA Repair: A Practical Approach, Methods in Pharmacology and Toxicology, doi: 10.1007/978-1-4939-1068-7_1, © Springer Science + Business Media New York 2014
Special issues
Mutation Research, Vol. 845, Sept 2019: Comet assay in vitro
Edited by Maria Dusinska, Solange Costa, Andrew R. Collins
Mutation Research, Vol. 843, July 2019: The Comet assay – Human biomonitoring
Edited by Andrew Collins, Mirta Milic, Stefano Bonassi, Maria Dusinska
Mutagenesis Vol. 33, Jan 2018: Special Issue - The comet assay
Guest Editor: Peter Møller
The articles in this Special Issue of Mutagenesis cover important technical issues of the comet assay procedure, nanogenotoxicity and ionising radiation sensitivity on plant cells. The included biomonitoring studies have assessed seasonal variation and certain predictors for the basal level of DNA damage in white blood cells. Lastly, the comet assay has been used in studies on genotoxicity of environmental and occupational exposures in human biomonitoring studies and animal models. Overall, the articles in this Special Issue demonstrate the versatility of the comet assay and they hold promise that the assay is ready for the next 30 years.
Frontiers Research Topics, 2015: 30 years of the Comet Assay: an overview with some new insights
Edited by Amaya Azqueta, Andrew Collins and Sabine Langie
Even after 30 years, the comet assay is still in a growth phase, with many new users each year. Many questions are repeatedly raised, which may seem to have self-evident answers, but clearly, it is necessary to reiterate them for the benefit of the new audience, and sometimes being forced to think again about old topics can shed new light. Different applications of the comet assay are discussed, including: genotoxicity testing, human biomonitoring, DNA repair studies, environmental biomonitoring and clinical studies. Furthermore, we will consider and where possible answer questions, including the ones raised by Raymond Tice at the 8th International Comet Assay Workshop in Perugia (Italy 2009): i) What is the spectrum of DNA damage detected by the various versions of the Comet assay?; ii) What are the limitations associated with each application?; iii) What should be done to standardize the assay for biomonitoring studies?; iv) Can the Comet assay be used to monitor changes in global methylation status?; What are the best cell types to use for detecting genotoxic substances in vitro?; v) Can the assay be fully automated?; and more. So this ‘Frontiers in Genetics’ research topic was written for the beginner as well as for the experienced users of the Comet Assay.
Mutagenesis Vol. 30, Jan 2015: Special Issue - The comet assay
Guest Editor: Andrew Collins
-
The comet assay: a heavenly method!
-
New developments in comet-FISH
-
Micropatterned comet assay enables high throughput and sensitive DNA damage quantification
-
Does the duration of lysis affect the sensitivity of the in vitro alkaline comet assay?
-
Comparison of DNA damage by the comet assay in fresh versus cryopreserved peripheral blood mononuclear cells obtained
following dietary intervention -
Recommendations for increasing alkaline comet assay reliability in plants
-
Experiences with the in vivo and in vitro comet assay in regulatory testingA repair after X-irradiation: lessons from plants
-
Effect of silver nanoparticles on mitogen-activated protein kinases activation: role of reactive oxygen species and implication in DNA damage
-
Applications of the comet assay in particle toxicology: air pollution and engineered nanomaterials exposure
-
Critical factors to be considered when testing nanomaterials for genotoxicity with the comet assay
-
The comet assay in Environmental Risk Assessment of marine pollutants: applications, assets and handicaps of surveying genotoxicity in non-model organisms
Mutagenesis Vol. 23, May 2008: Special Issue on the comet assay
Guest Editor: Valerie McKelvey-Martin
-
The comet assay: topical issues
-
The use of the comet assay in the study of human nutrition and cancer
-
Using the alkaline comet assay in prognostic tests for male infertility and assisted reproductive technology outcomes
-
Statistical issues in the use of the comet assay
-
Potential use of the comet assay in the clinical management of cancer
-
The comet assay in human biomonitoring: gene–environment interactions
-
Ecotoxicological applications and significance of the comet assay
-
Variation in assessment of oxidatively damaged DNA in mononuclear blood cells by the comet assay with visual scoring
-
Recommendations for design of the rat comet assay
Meeting reports & Abstracts
Reports
ICAW 2019: Gajski G, Langie S, Zhanataev A. Recent applications of the Comet Assay: A report from the International Comet Assay Workshop 2019. Toxicol Lett. 2020 Oct 15;333:1-3. https://doi.org/10.1016/j.toxlet.2020.07.022
ICAW 2015: Koppen G, Azqueta A, Pourrut B, Brunborg G, Collins AR, Langie SAS. The next three decades of the comet assay: a report of the 11th International Comet Assay Workshop. Mutagenesis. 2017 May 1;32(3):397-408. https://doi.org/10.1093/mutage/gex002
ICAW 2001: Hartmann A, Agurell E, Beevers C, Brendler-Schwaab S, Burlinson B, Clay P, Collins A, Smith A, Speit G, Thybaud V, Tice RR; 4th International Comet Assay Workshop. Recommendations for conducting the in vivo alkaline Comet assay. 4th International Comet Assay Workshop. Mutagenesis. 2003 Jan;18(1):45-51. https://doi.org/10.1093/mutage/18.1.45
ICAW 1997: Anderson D, Plewa MJ. The International Comet Assay Workshop. Mutagenesis. 1998 Jan;13(1):67-73. https://doi.org/10.1093/mutage/13.1.67
Abstracts
Abstracts of the 12th International Comet Assay Workshop held at the University of Navarra, Pamplona, Spain, 29-31 August 2017 (https://icaw.vito.be/). Mutagenesis. 2018 Sep 21:341. https://doi.org/10.1093/mutage/gex037
11th International Comet Assay Workshop published in Frontiers in Genetics
Abstracts from the 7th International Comet Assay Workshop held at University of Ulster, Coleraine, Northern Ireland; 24th 27th June 2007. (2007). Mutagenesis, 22(6), e1–e21.doi:10.1093/mutage/gem033