One hour is generally regarded as the minimum required. It is also a convenient time in which to go to have lunch! Overnight is OK, unless you have a problem with gel attachment, in which case the extended lysis can cause losses.
"FAQ by Andrew Collins, Gunnar Brunborg and Jonas Nygren, 2006, NewGeneris FP7-project"
One hour is generally regarded as the minimum required. It is also a convenient time in which to go to have lunch! Overnight is OK, unless you have a problem with gel attachment, in which case the extended lysis can cause losses.
"FAQ by Andrew Collins, Gunnar Brunborg and Jonas Nygren, 2006, NewGeneris FP7-project"