No. Mitochondrial DNA molecules are small (about 17 kb) and are – of course – not attached to the nuclear matrix. They have been visualised by fluorescent in situ hybridisation with ‘padlock’ probes (Shaposhnikov et al. (2006), Mutagenesis 21, 243-7) and appear clustered around the nucleus of embedded cells, but as soon as lysis starts, the mtDNA begins to disperse, and by the time electrophoresis begins virtually no mtDNA molecules are left.
"FAQ by Andrew Collins, Gunnar Brunborg and Jonas Nygren, 2006, NewGeneris FP7-project"