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Why do I sometimes see images resembling halos, rather than comets, even when there is sufficient damage to produce tails? icaworkgroup Dash Core Group Question	1	0	May 11

Do I need to buy image analysis software? icaworkgroup Dash Core Group Question	1	0	May 11

What is the best image analysis parameter to use? icaworkgroup Dash Core Group Question	1	0	May 11

Can the comet assay be used to measure DNA repair? icaworkgroup Dash Core Group Question	1	0	May 11

How do I measure other kinds of damage, in addition to strand breaks? icaworkgroup Dash Core Group Question	1	0	May 11

Can the comet assay be used to detect damage in mitochondrial DNA? icaworkgroup Dash Core Group Question	1	0	May 11

Can the comet assay be used to detect apoptotic cells? Are ‘hedgehog’ comets a sign of apoptosis? icaworkgroup Dash Core Group Question	1	0	May 11

Does it matter if there are non-viable cells among those used in the comet assay? What % is acceptable? icaworkgroup Dash Core Group Question	1	0	May 11

How important is current? icaworkgroup Dash Core Group Question	1	0	May 11

How important is voltage? icaworkgroup Dash Core Group Question	1	0	May 11

How important is lysis time? icaworkgroup Dash Core Group Question	1	0	May 11

How important is pH? icaworkgroup Dash Core Group Question	1	0	May 11

How can I prevent gels from falling off the slides? icaworkgroup Dash Core Group Question	1	0	May 11

Do you need to use alkaline conditions to see single strand breaks? icaworkgroup Dash Core Group Question	1	0	May 11

Does the neutral comet assay only detect double strand breaks? icaworkgroup Dash Core Group Question	1	0	May 10

General Discussions

Why do I sometimes see images resembling halos, rather than comets, even when there is sufficient damage to produce tails?

Do I need to buy image analysis software?

What is the best image analysis parameter to use?

Can the comet assay be used to measure DNA repair?

How do I measure other kinds of damage, in addition to strand breaks?

Can the comet assay be used to detect damage in mitochondrial DNA?

Can the comet assay be used to detect apoptotic cells? Are ‘hedgehog’ comets a sign of apoptosis?

Does it matter if there are non-viable cells among those used in the comet assay? What % is acceptable?

How important is current?

How important is voltage?

How important is lysis time?

How important is pH?

How can I prevent gels from falling off the slides?

Do you need to use alkaline conditions to see single strand breaks?

Does the neutral comet assay only detect double strand breaks?